Day 2 :
- POSTER PRESENTATION
Location: Zurich, Switzerland
Session Introduction
Apichaya Puangpetch
Mahidol University, Thailand
Title: Development and validation of LC-MS-MS method for determination of ibuprofen in human plasma
Time : 11:00-12:00
Biography:
Apichaya Puangpetch has completed her PhD from Khon Kaen University and Postdoctoral studies from Mahidol University. She is the Lecturer of Pharmacogenomics in Mahidol University. She has published more than 40 papers in reputed journals and has been studing the TPMT enzyme activity and NUDT polymorphisms.
Abstract:
Ibuprofen is widely used to reduce fever, pain or inflammation. Moreover, one of the most aspects of ibuprofen usage is for the closure of the patent ductus arteriosus (PDA) in preterm infant. To study pharmacokinetics in human, the method for drug determination is required to accurately and reliably appropriate strategy to predict the rate of PDA closure. From the previous review the researchers found that the high area under the curve of oral ibuprofen contributes to better rates of successful PDA closure. Liquid chromatography with tandem mass spectrometry (LC-MS-MS) is the one of the bioanalytical methods that has the highly sensitivity, accuracy, reliable, fast as well as simple. In this study, we conducted the LC-MS-MS method for ibuprofen bioavailability. Samples were stable at room temperature in autosampler for 24 h. The calibration curve was linear across the concentration range of 0.15-50 mg/ml. The coefficient of variation for intra-day and inter-day precision was 0.78-7.21% and accuracy was within 97.52-107.21 of the nominal values for QCL (0.45 mg/ml), QCM (9.0 mg/ml) and QCH (40.0 mg/ml). For ibuprofen concentration at the lower limit of quantification (LLOQ), intra-day and inter-day accuracy of the LLOQ was 98.11% and 99.81% while the intra-day and inter-day precision were 1.89% and 5.37%. Recovery was 84-94%. The pharmacokinetic study in 18 neonates which was divided into low and high dose group was analyzed. The median maximum concentration of ibuprofen for low and high regimen was 16.05 (14.21-19.32) and 24.10 (20.63-32.03) µg/ml, the median elimination rate constant (ke) was 0.041 (0.026-0.047) and 0.071 (0.050-0.073) hr-1, respectively. Therefore, LC-MS-MS method was a suitable technique to the analysis of unknown plasma samples for ibuprofen pharmacokinetic, bioavailability or bioequivalence studies.
Putthapoom Lumjiaktase
Mahidol University, Thailand
Title: Suppressive activity method of human peripheral blood regulatory T Cells in rheumatoid arthritis patients
Biography:
Putthapoom Lumjiaktase is an Instructor in Clinical Pathology at Faculty of Medicine Ramathibodi Hospital, Mahidol University. He graduated BSc in Medical Technology, MSc and PhD in Clinical Pathology at Mahidol University, Thailand and Postdoctoral fellowship at University of Zurich, Switzerland. He have been involved in diverse topics as Immunology, Clinical pathology, Molecular Microbiology and Ecology.
Abstract:
Regulatory T cell (Treg) is an immune suppressor. Mechanisms of its suppression include inhibitory cytokine secretion, expression of inhibitory molecules and apoptosis induction by perforin and gramzyme. The number of peripheral blood (PB) Treg in rheumatoid arthritis (RA), a chronic inflammatory autoimmune disease, is impaired due to the disease state. Yet, current evidences are insufficient to indicate the immune status of RA patients owing to T cell plasticity property. Suppression assay is a functional study used to determine Treg suppressive activity. Because conventional protocol requires an extensive Treg expansion in vitro prior to experiment, this study aims to establish a co-culture suppression assay to determine the suppressive activity of Treg derived from PB of RA patients by using short-term expanded PB-Treg, requiring no freeze/thaw autologous conventional T cell (Tconv) for the assay. Treg of RA patients were isolated from peripheral blood mononuclear cells in two steps: initial CD8+ depletion by magnetic sorting and cell sorting by FACSAria III. Treg underwent short-term expansion while autologous Tconv was rested by resting assay. Later, Treg and Tconv were co-cultivated for 3 days and suppressive function was measured by monitoring of CFSE division using flow cytometry. Tconv proliferation in absence of Treg was about 50-60% while in presence of Treg was 13-30%. In control group, such percentage of suppression was about 64%. Significantly, suppression activity (%) of Treg differs between active and remission RA patients.
Korawit Kanjana
Mahidol University, Thailand
Title: Suppressive activity method of human peripheral blood regulatory T Cells in rheumatoid arthritis patients
Biography:
Korawit Kanjana is a PhD candidate in Clinical Pathology program at Faculty of Medicine Ramathibodi Hospital, Mahidol University and completed for certificate online course in Design and Interpretation of Clinical Trials program from Johns Hopkins Bloomberg School of Public Health, Johns Hopkins University. He has completed his bachelor degree from Walailak University. He had been nominated finalist for Merck Young Scientist Award 2015.
Abstract:
Regulatory T cell (Treg) is an immune suppressor. Mechanisms of its suppression include inhibitory cytokine secretion, expression of inhibitory molecules and apoptosis induction by perforin and gramzyme. The number of peripheral blood (PB) Treg in rheumatoid arthritis (RA), a chronic inflammatory autoimmune disease, is impaired due to the disease state. Yet, current evidences are insufficient to indicate the immune status of RA patients owing to T cell plasticity property. Suppression assay is a functional study used to determine Treg suppressive activity. Because conventional protocol requires an extensive Treg expansion in vitro prior to experiment, this study aims to establish a co-culture suppression assay to determine the suppressive activity of Treg derived from PB of RA patients by using short-term expanded PB-Treg, requiring no freeze/thaw autologous conventional T cell (Tconv) for the assay. Treg of RA patients were isolated from peripheral blood mononuclear cells in two steps: initial CD8+ depletion by magnetic sorting and cell sorting by FACSAria III. Treg underwent short-term expansion while autologous Tconv was rested by resting assay. Later, Treg and Tconv were co-cultivated for 3 days and suppressive function was measured by monitoring of CFSE division using flow cytometry. Tconv proliferation in absence of Treg was about 50-60% while in presence of Treg was 13-30%. In control group, such percentage of suppression was about 64%. Significantly, suppression activity (%) of Treg differs between active and remission RA patients.